This study was carried out to isolate and identify the causal fungus of apple rot, and to examine the nutrients and pH ranges on mycelial growth of the fungus.
The summarized results were as follows;
1. The causal fungus isolated from infected apple twigs and fruits was identified as Botryosphaeria dothidea in teleomorph and Dothiorella mall in anamorph, and their pathogenicity was tested by wound inoculations.
2. The pathogen grew well on potato sucrose agar, and also on oat meal sucrose agar. The optimum pH of medium ranged 5.0∼6.0 and the highest survival temperature of the fungus was approximately 55℃.
3. Out of series trials investigating the effects of different nutrient sources on mycelial growth, starch, sucrose, and fructose as carbon sources and sodium nitrate, urea, and serine as nitrogen sources were effective. The addition of ascorbic acid to the basal medium also prompted the mycelial growth of the fungus among various vitamins sources.
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